Effects of Two Plant Growth Promoting Bacteria and Their Mixture on the Growth of Fast-growing Chinese Cabbage 888
In order to find a mixed microbial agent with high growth promoting efficiency for crops,this study used two strains(L1 13 and L35)selected from the Shijiao mangrove forest in Guangxi to establish a mixed micro-bial agent using molasses waste as the carrier.The optimal mixed bacterial formula was screened using molybde-num antimony resistance colorimetric test;determine the optimal fermentation conditions for the composite strain through orthogonal experiments;validate the growth promoting effect of mixed strains on 888 fast-growing cab-bage through pot experiments.The results of molybdenum antimony resistance experiment showed that when the ratio of strain L1 13 and L35 was 1∶1(volume ratio),and the inoculation amount was 3％(volume ratio),the mixed strain prepared had a phosphorus solubility of 89.12 mg/L.Under these conditions,the phosphorus content of the mixed strain prepared was the best.The orthogonal test results showed that the optimal fermentation conditions for the prepared mixed strain were temperature 30 ℃,pH=7.5,and fermentation time of 72 hours.The experimental verification showed that the mixed bacterial count of the composite strain was 1×109 CFU/mL.The results of the pot experiment showed that the mixture of strains L1 13 and L35 had a good growth promoting effect on Chinese cabbage.The plant height,fresh weight,dry weight,and leaf number of Chinese cabbage seedlings treated with the mixed bacterial liquid were significantly increased by 19.35％,24.22％,33.21％,and 28.60％compared to the clean water control(CK1);and 4.22％,16.50％,22.22％,and 16.36％,respectively,compared to the sterile cul-ture medium control(CK2).This indicates that the growth status of Chinese cabbage cultured with the mixed bacterial liquid is better than the control group.This experiment developed a mixed bacterial agent using molasses as the culture medium,providing strain formula and important experimental data support for the further industrial production and promotion of the composite bacterial agent.
Mixed strainsMolasses alcohol wastewaterFermentation system constructionChinese cabbage grows
NETL
NSTL
万方数据
