Comprehensive Evaluation of Salt Tolerance in Cuphea hookeriana Based on Principal Component Analysis and Pathway Analysis
In order to investigate the tolerance of Cuphea hookeriana to soil salinization,C.hookeriana cuttings were used as experimental materials to simulate salt stress by the potting method,which was divided into five concentration gradient treatments,namely,0,25,50,75,and 100 mmol/L,and the changes of various physiological indexes were determined.The results of the study revealed that relative conductivity,proline content,and malondialdehyde content of C.hookeriana were positively correlated with salt stress concentration;relative water content was negatively correlated with it;and both chlorophyll a and carotenoids showed a decrease followed by an increase in salt stress conditions,while chlorophyll b showed a decrease followed by an increase followed by a decrease in salt stress conditions.It was found that C.hookeriana had different physiological responses to different concentrations of stress,and the higher the concentration of stress,the greater the effect on acteristics of seedlings;C.hookeriana could respond to salt stress by decreasing the relative water content and in-creasing the content of malondialdehyde and proline;furthermore,the content of chlorophyll a,chlorophyll b,and carotenoids could be rebounded to a certain extent after a decrease to reduce physiological disorders caused by salt stress.The effects on the physiological characteristics of C.hookeriana were found to be 100,75,25,50,and 0 mmol/L in descending order by principal component analysis,and the most important factor affecting the proline content of C.hookeriana under salt stress was found to be the relative water content by pass-through analysis.The present study investigated the physiological response mechanism of C.hookeriana under NaCl salt stress,which is of great significance for the enhancement of salt tolerance and wider introduction of C.hookeriana into cultivation.
Cuphea hookerianaNaCl salt stressPhysiological characteristicsPathway analysisPrincipal com-ponent analysis
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