Effects of Synchytrium puerariae Miy on Defense Enzymes and Malondial-dehyde in Pueraria
Pueraria pseudo-rust is a serious disease that restricts the development of pueraria industry in recent years,which leads to the decrease of the yield and quality of pueraria varieties year by year,and causes great economic losses to growers.Pueraria pseudo-rust is a fungal disease caused by Synchytrium puerariae Miy.Defensive enzyme and malondialdehyde(MDA)are important biochemical indexes of host defense response and resistance ability.The sensitive cultivar'Guige8'and resistant cultivar'Guige18'which selected by our research group were used as test materials to study the activities of superoxide dismutase(SOD),polyphenol oxidase(PPO),catalase(CAT)and chitinase(CHI),as well as malondialdehyde(MDA)content changes by artificial living inoculation.The results showed that the activities of four defense enzymes and MDA content of the two pueraria varieties changed significantly after being infected by Synchytrium puerariae Miy.The CAT,PPO,SOD and CHI defense enzyme activities of the resistant cultivar'Guige18'were significantly higher than those of the sensitive cultivar'Guige8'in both the control group and the treatment group,with a greater increase rate and a longer duration at a higher activity level.On the contrary,the MDA content of'Guige8'was higher than that of'Guigel8'significantly and maintained at a higher level for a longer time.There were significant differences in the defense enzymes activities and MDA contents between the two varieties of pueraria.The results showed that there was a certain correlation between defense enzyme and disease resistance of pueraria.The difference of response speed to pathogen infection reflected the degree of resistance of pueraria varieties to Synchytrium puerariae Miy.Compared with the sensitive cultivar,the resistant one had faster response speed and higher resistance activity to Synchytrium puerariae Miy infection.
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