摘要
为探讨小粒种咖啡的耐寒机制,以1年生小粒种咖啡苗为试材,设置8 ℃、4 ℃、0 ℃、-2 ℃ 4个低温胁迫处理,研究不同温度对其植株、叶片光合参数和叶绿素荧光参数的影响.结果表明:随着温度的降低和处理时间的延长,小粒种咖啡植株寒害程度不断加剧,叶片叶绿素含量、光合参数的Pn、Gs、Tr、WUE、Ls以及叶绿素荧光参数的ΦPSⅡ、qP和ETR整体呈先升后降,Ci、Fo是上升趋势,Fv/Fm则逐渐降低.相关性分析表明,Chl、Pn、WUE、Ls、ETR、qP、qN等指标对咖啡抗寒起正向作用,Ci、Tr与其他指标呈负相关,起负作用.综上所述,低温胁迫抑制了小粒种咖啡苗期叶片光合作用及削弱叶绿素荧光特性,该研究结果为筛选咖啡优异抗寒种质提供参考,并为开展抗寒分子机理研究提供理论依据.
Abstract
To investigate the mechanism of cold-resistance in Coffea arabica,one-year-old Coffea arabica plants were treated by four different low temperatures of 8 ℃,4 ℃,0 ℃,-2 ℃ were used to study the effects of photo-synthetic parameters and chlorophyll fluorescence parameters in leaves.The results showed that the degree of chilling injury of Coffea arabica plants is increasing with the decrease of temperature and the extension of treat-ment time,chlorophyll(Chl)content,net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(Tr),water use efficiency(WUE),stomatal limit value(Ls)of photosynthetic parameters,and actual photochemical quantum yield(ΦPSⅡ),photochemical quenching coefficient(qP)and quantum transfer rate(ETR)of chlorophyll fluorescence parameters as a whole were declined after rising.The intercellular CO2 concentration(Ci)and initial fluorescence(Fo)are increasing,while the maximal photochemical yield(Fv/Fm)decreases gradually.Correlation analysis showed that Chl,Pn,WUE,Ls,ETR,qP and qN of coffea was positive correlated with cold resistance,but the negative correlation between the Ci,Tr and the others.The chlorophyll fluorescence characteristics of sorghum seedlings were weakened by low-temperature stress treatment.The results would provide reference for the screen-ing excellent cold resistant germplasm and resistance molecular mechanism research of coffee.
基金项目
海南省自然科学基金项目(322QN405)
国家现代农业产业技术体系项目(CARS—11)
云南省科技厅院士专家工作站项目(202205AF150080)
NETL
NSTL
万方数据