Advance in Tissue Culture Techniques and Applications of Tea Plant(Ca-mellia sinensis)
Camellia sinensis,a perennial plant with cross-pollination characteristics,possesses significant eco-nomic and beverage value.The application of plant tissue culture technology offers an effective approach for vari-ous facets of tea plant cultivation,including rapid propagation,germplasm preservation,genetic transformation,polyploid breeding,gene function investigation,and the production of secondary metabolites.This review provides an overview of tea plant tissue culture technology,with a specific emphasis on factors influencing proliferation and rooting.Furthermore,it addresses prevalent challenges encountered during tea plant tissue culture,such as issues related to browning and contamination.Additionally,the review offers a comprehensive examination of the prima-ry applications of tissue culture in tea plant cultivation.This study provides a reference for the improved utilization of tea plant germplasm resources.