Expression and Functional Analysis of the Sweet Sorghum Sucrose Transporter SUT5
To reveal the functions of sweet sorghum sucrose transporter SdSUT5 gene and its expression patterns under different abiotic stresses,a sweet sorghum SUT5 gene was obtained by homologous cloning and bioinfor-matics and subcellular localization analysis was performed in this study.The real-time fluorescence quantitative PCR method was used to study the response characteristics of SdSUT5 gene in different tissues under four abiotic stresses:low temperature,high temperature,salt and drought,and at the same time the function of the SdSUT5 gene was verified by transforming the hexose transporter-deficient yeast EBY.VW4000.Results of bioinformatics analysis showed that the gene was 1 602 bp in length,encoding 534 amino acids,the theoretical isoelectric point was 8.63,and belongs to a stable hydrophilic protein.There were 12 transmembrane regions and 43 phosphory-lation sites;the secondary structure was dominated by α-helix and random coil.The experiment of subcellular localization showed that SdSUT5 protein was localized at the plasma membrane.The hexose transporter-deficient yeast EBY.VW4000 was used to demonstrate that theprotein encoded by SdSUT5 gene has the function of trans-ferring sucrose.The phylogenetic tree showed that the sweet sorghum SUT5 protein was more closely related to sugarcane and maize.Real-time fluorescence quantitative PCR analysis showed that the SUT5 gene responded with different degrees in different parts of sweet sorghum after suffering stresses of low temperature,high temperature,drought and salt.The analysis of cloning and function of the SdSUT5 gene in sweet sorghum has provided theoretical references for breeding sweet sorghum for stress resistance.
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