Identification and Expression Analysis of Oil Biosynthesis Related WRI1s Genes in Oil Palm
WRI1 genes play an important regulatory role in palm oil synthesis,this study aims to explore WRI1 genes and analyze its physical and chemical properties and tissue expression characteristics.Using bioinformatics method,a total of 3 EgWRI1 genes were found,all of which contained 6 introns.Through chromosome mapping analysis,it was found that EgWRI1-1 and EgWRI1-2 were located on chromosome 5 of oil palm,and EgWRI1-3 was located on chromosome 10 of oil palm.EgWRI1-1,EgWRI1-2 and EgWRI1-3 genes all contain AP2 domains,encoding 337,338 and 367 aa,respectively.The isoelectric points of EgWRI1-1 and EgWRI1-3 were less than 7,which were 6.51 and 6.29,respectively,and that of EgWRI1-2 were more than 7(8.31).They were hydrophilic proteins,no signal peptide,contain multiple phosphorylation sites.They exited in nucleus by subcellular localization analysis.The main structure of EgWRI1-1 and EgWRI1-3 was α-helix,EgWRI1-2 was random coil.Cluster analysis showed that at the genetic coefficient of 0.625,three EgWRI1 and AtWRI1 were in the same branch.At the genetic coefficient of 0.680,EgWRI1-1 and EgWRI1-2 had the closest evolutionary relationship and clustered with EgWRI1-3.Quantitative PCR analysis showed that the expression level of EgWRI1-1 was the highest at 80 day in mesocarp,the expression level of EgWRI1-2 was the highest at 95 day in endosperm,and EgWRI1-3 was not expressed in all tissues.The results laid a foundation for further study on the regulation me-chanism of EgWRI1-1 and EgWRI1-2 in the process of fatty acid synthesis in mesocarp and endocarp of oil palm.
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