Gene Cloning and Prokaryotic Expression of Catalase in Cassava
The dynamic changes of reactive oxygen species(ROS)play essential roles in stress response of plants.Catalase(CAT)is the one of the key enzymes regulating ROS homeostatic in animals and plants.To better investi-gate the genetic characteristic and the role of MeCAT1 in response to the changes of external environment in cas-sava,the MeCA T1 was identified and cloned,and the prokaryotic expression vector of MeCAT1 was constructed in this experiment.Protein domain analysis indicated that MeCAT1 has the conserved protein domain of catalase.Then,MeCAT1 recombinant protein showed significant CAT enzyme activity through protein induction and en-zyme activity measurement.These results provides a certain molecular basis for further exploring the role of MeCAT1 in response to biotic and abiotic stresses,and it is helpful for the deep excavation and innovative utiliza-tion of MeCAT1 in cassava.
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