Identification of Actinidia chinensis Expansin Gene Family and Expression under Different Stresses
Expansin is an important part of cell wall,with loosing cell wall components,increasing cell wall flexibility function.It widely exists in the genomes of different plants.In this study,based on the genome sequence of Actinidia chinensis'Hongyang',obtained the expansin sequence information of Actinidia chinensis by using the characteristic region comparison of Populus trichocarpa expansin sequences.Based on the bioinformatics and codon analysis software,obtained the Actinidia chinensis expansin gene family characteristics and codon preference information.Whole Actinidia chinensis expansin family contains 39 expansin genes,which belong to 4 subfamilies AcEXPA(28),AcEXPB(6),AcEXPLA(1)and AcEXPLB(4),of which 36 members are located in 22 chromosomes.Amino acid composition analysis showed that Gly content was the highest(11.74％),while His content was the lowest(1.91％).Analysis of codon characteristics showed that the third base of Actinidia chinensis expansin preferred pyrimidine,and the value of sequence GC tended to be used equally.Through the analysis of synonymous codon usage frequency,study found that there are 8 high frequency codons,which are with the end of base G/C.3,14,7 and 6 genes were involved in the response to low temperature,high temperature,drought and salt stress respectively.The identification,codon usage and function analysis of Actinidia chinensis expansin gene family is helpful to understand its composition and efficiency in Actinidia chinensis genome.It also provides a basis for the study of the role of expansin in kiwifruit and heterologous expression in the future.
Actinidia chinensisExpansinGene familyCodon usageGene function
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NSTL
万方数据
