Identification of Endophytic Fungi Species from Cypripedium shanxiense
This study analyzed the species of endophytic fungi in Cypripedium shanxiense in Shanxi Province,and compared with earlier reports about C.shanxiense from other regions distribution to explore the correlation be-tween geographic distribution and the species of endophytic fungi in C.shanxiense.Through sequencing the ITS gene fragments of endophytic fungi and downloading similar sequences from GenBank,an NJ phylogenetic tree was constructed.Based on the principle of similarity,the endophytic fungi were identified molecularly.The results showed that the endophytic fungi in Cypripedium shanxiense include 11 representative taxa belonging to 4 classes:Agaricomycetes(36％),Dothideomycetes(28％),Sordariomycetes(18％),Eurotiomycetes(18％);5 orders:Poly-porales(28％),Agaricales(9％),Pleosporales(18％),Hypocreales(9％),Eurotiales(18％),not assigned order(18％).There is a certain difference in the species of endophytic fungi in C.shanxiense compared with other regions,indicating that geographic distribution has a certain correlation with the composition of endophytic fungal species.
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