Tryptophan synthase subunit(TSA)plays an important role in plant growth and development by contro-lling the indole level in plants through the cleavage of indole glyceryl phosphate aldehyde to indole.In order to ex-plore the response mechanism of maize TSA gene family to stress,this study screened and identified the whole genome data of B73-REFERENCE-GRAMENE-4.0 version of maize,and identified 8 members of TSA family,which were located on 4 chromosomes,and were distributed unevenly.The bioinformatics analysis found.The length,exons and introns of ZmTSA gene were different,there were 9 conserved motifs,two of which were com-mon to all members,and ZmTSA gene could be divided into two groups.The ZmTSA gene is located in the nucle-us,cytoplasm and chloroplast,and the secondary structure is composed of α-helix and random coil.,and most of its members have similar tertiary structures.The ZmTSA family is regulated by various stresses and exhibits di-verse expression patterns during growth and development.This study provides a theoretical basis for the follow-up study of ZmTSA gene family.