玉米TSA基因家族生物信息学分析
Bioinformatics Analysis of Maize TSA Gene Family
张敏艳 1黄伟鹏 1徐碧莹 1郑大浩 1吴委林1
作者信息
摘要
色氨酸合酶α亚基(tryptophan synthase alpha chain,TSA),通过将吲哚磷酸甘油酯醛裂解为吲哚,调控植物体内的吲哚水平,对植物生长发育起重要的作用.为探究玉米TSA(ZmTSA)基因家族对胁迫的响应机制,本研究对B73-REFERENCE-GRAMENE-4.0版玉米基因组数据进行筛选鉴定,共鉴定出8个TSA家族成员,定位于4条染色体上,不均等分布.ZmTSA基因长度、外显子、内含子数量差异较大;基因有9个保守基序,其中两个保守基序为所有成员所共有;ZmTSA基因可以分为两个类群;ZmTSA基因定位于细胞核、细胞质和叶绿体上,二级结构主要由α-螺旋和无规则卷曲组成,多数成员三级结构相似;ZmTSA家族受多种胁迫调控,生长发育过程中呈多种表达模式.本研究为ZmTSA基因家族后续的研究提供理论依据.
Abstract
Tryptophan synthase subunit(TSA)plays an important role in plant growth and development by contro-lling the indole level in plants through the cleavage of indole glyceryl phosphate aldehyde to indole.In order to ex-plore the response mechanism of maize TSA gene family to stress,this study screened and identified the whole genome data of B73-REFERENCE-GRAMENE-4.0 version of maize,and identified 8 members of TSA family,which were located on 4 chromosomes,and were distributed unevenly.The bioinformatics analysis found.The length,exons and introns of ZmTSA gene were different,there were 9 conserved motifs,two of which were com-mon to all members,and ZmTSA gene could be divided into two groups.The ZmTSA gene is located in the nucle-us,cytoplasm and chloroplast,and the secondary structure is composed of α-helix and random coil.,and most of its members have similar tertiary structures.The ZmTSA family is regulated by various stresses and exhibits di-verse expression patterns during growth and development.This study provides a theoretical basis for the follow-up study of ZmTSA gene family.
关键词
玉米/生物信息学/TSAKey words
Maize/Bioinformatics/TSA引用本文复制引用
基金项目
国家自然科学基金项目(31660395)
吉林省教育厅科学技术研究项目(JJKH20210574)
吉林省科技厅基础研究专项(202002026JC)
延边大学校项目(延大科合字2020第9号)
出版年
2024
NETL
NSTL
万方数据