Cloning and Expression Characteristics of Zinc Finger Protein Gene ZmRFP from Maize(Zea mays L.)
Zinc finger protein(ZFP)is a kind of important transcription factor,which regulates gene expression by interacting with nucleic acid,it plays an important role in plant growth,senescence and abiotic stress.In this study,we cloned a C3H2C3 zinc finger protein gene(ZmRFP)from maize inbred line'Liao3162'.The open reading frame length of ZmRFP was 1 563 bp,encoding a protein containing 520 amino acids.The predicted molecular weight was 57.06 kD,and the isoelectric point was 6.18.Analysis of conservative domain and transmembrane domain showed that the protein belonged to zinc finger protein family transcription factor with RING domain,it was also an non-secretory transmembrane domain protein.The amino acid residues were mainly random CRIMP based on prediction of the secondary structure and the tertiary structure.There were several components related to abiotic stress and plant hormones in the upstream 2 000 bp.Phylogenetic analysis showed that the evolution of RFP pro-teins was highly conservative and most similar to that of sorghum.Organ specific analysis showed that the expres-sion of this gene was the highest in maize leaves.Under abiotic stress,the gene expression was induced under salinity and drought stress.Cloning and expression analysis of ZmRFP gene in maize laid a foundation for further study of its biological function and application.
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