Transcriptome Analysis and Flavonoid Gene Analysis of Gynura formosana Kitam.
Gynura formosana Kitam.is a famous Chinese herbal medicine in southern Fujian and Taiwan,China.Its functions are diverse,but there is little research in recent years,especially molecular research.This study selected Illumina HiSeq™ the platform carried out high-throughput sequencing on the leaves and stems of G.formosana,and the research clarified that a total of 93 975 high-quality unigenes were spliced,with an N50 of 1146 bp and an average length of 624.78 bp;compared with seven major databases such as GO database,56 455 unigenes annotated at least one database successfully,and 2 005 unigenes annotated all.A total of 3 286 unigenes were obtained in the differential expression of roots in the leaves of G.formosana,of which 1 852 were expressed with an upward trend and 1 434 were expressed with a downward trend.Using the KEGG database,39 unigenes were found to be involved in flavonoid biosynthesis,with 11 showing significant differences,distributed among 12 synthetases.These results provide a theoretical basis for further research on the molecular mechanism of biosyn-thesis and regulation of flavonoids in G.formosana.
NETL
NSTL
万方数据
