Identification and Expression Analysis of PIN Genes Family in Pepper
In this study,the PIN gene family in pepper was identified and functionally analyzed by means of bioinformatics,and the differential expression characteristics of CaPINs genes were explored through abiotic stress.The results showed that there were 5 members of the PIN family in pepper,which could be divided into 3 subgroups,and all contained the conserved domains of Mem-trans and XPXXY;they were mainly distributed on 4 chromosomes.They all have signal peptides,which are hydrophobic proteins,and are most likely to be located in the cell membrane.They are all transmembrane proteins.Motif analysis found that the N-terminal was mainly mo-tif 3,and the C-terminal was mainly motif 1,which are highly conserved in the sequence and have complete sec-ondary structure.The phylogenetic tree analysis showed that the amino acid sequence encoded by this gene was most closely related to tomato.The results of RT-qPCR analysis showed that under NaCl stress,the relative expres-sion levels of CaPIN1 and CaPIN3～CaPIN5 in pepper leaves were up-regulated,while the relative expression levels of CaPIN2 in pepper leaves were down-regulated,and the difference was significant.Under PEG and LT stress,the relative expression levels of CaPIN1～CaPIN5 in pepper leaves were up-regulated,and the difference was significant.Different expressions of CaPIN family genes indicate that they play a potential role in abiotic stress.
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