椰枣幼苗地上部和根部显微结构观察和植物激素相关差异基因分析
Microstructure Observation and Hormone-related Differential Genes Analy-sis of the Date Palm Seedlings about Shoots and Roots
李东霞 1符海泉 1张宁 1王义 1徐中亮1
作者信息
- 1. 中国热带农业科学院椰子研究所,海南省热带油料生物学重点实验室,国家热带棕榈种质资源圃,文昌,571339
- 折叠
摘要
椰枣是干旱和半干旱地区重要的木本粮食作物之一,其地上部和根部形态显微结构差异明显,然而内在的差异转录机制不清楚.为探明椰枣幼苗地上部和根部差异机制,本研究对椰枣幼苗的地上部和根部进行转录组测序,重点分析植物激素信号转导通路的生长素、细胞分裂素、脱落酸、赤霉素、水杨酸和茉莉素相关基因.结果表明,地上部和根部形态与显微结构差异明显,叶片具有圆球形的叶肉细胞,茎中有较为密集的细胞核,根的中间为中柱.转录组分析发现,共有7 101个差异表达基因,上调3 042个,下调4 059个.差异表达基因的KEGG通路分析结果显示,前5条通路中的第2条途径为植物激素信号转导合成途径.地上部生长素TIR1,赤霉素GID1、DELLA、TF,水杨酸NPR1、PR-1基因高表达;而根部生长素AUX1、PIN,细胞分裂素AHP、B-ARR、A-ARR、B1N3,水杨酸TGA,茉莉素JAZ,脱落酸PP2C、ABF、SnRK2基因高表达.椰枣幼苗的地上部和根部的植物激素相关基因具有组织表达特异性,参与调控椰枣幼苗的发育机制不同,为后续外源激素调控椰枣幼苗地上部和根的生长发育提供基础.
Abstract
Date palm is one of the most important woody food crops in arid and semi-arid regions.The morpho-logical and microscopic structure of the shoot and root are different,while the transcription mechanism is unclear.In order to explore the mechanism of differences between the shoot and root of date palm seedlings,transcriptome sequencing was performed in this study,focusing on the analysis of genes related to auxin,cytokinin,abolic acid,gibberellin,salicylic acid and jasmonin in plant hormone signal transduction pathways.The results indicated that the morphological and microscopic structure of the shoot and root are different,the leaves have spherical meso-phyll cells,the stems have relatively dense nuclei,and the middle of the root is the stele.Transcriptome analysis revealed there are 7 101 differentially expressed genes,3 042 upregulated and 4 059 downregulated.KEGG path-way analysis of differentially expressed genes showed that the second pathway was a plant hormone signal trans-duction.Genes of auxin TIR1,gibbecellins GID1,DELLA,TF,and salicylic acid NPR1 and PR-1 were highly ex-pressed in shoot.However,the genes of auxin AUX1 and PIN,cytokinin AHP,B-ARR,A-ARR and BIN3 genes,salicylic acid TGA,jasmonic acid JAZ,abscisic acid PP2C,ABF and SnRK2 genes were highly expressed in root.The plant hormone-related genes in shoot and root of date palm seedlings have tissue-specific expression,and the mechanisms involved in the regulation of the development of date palm seedlings are different,which provides the basis for the subsequent regulation of the growth and development of shoot and root of date palm seedlings by ex-ogenous hormones.
关键词
椰枣/地上部/根/植物激素Key words
Phoenix dactylifera L./Shoot/Root/Plant hormone引用本文复制引用
基金项目
海南省自然科学基金项目(319QN325)
农业农村部财政专项-农业国际交流与合作(12516301500020-0006)
物种品种资源保护费项目(125163015000160004)
出版年
2024
NETL
NSTL
万方数据