Transcriptome Analysis and Mining of Genes Related to Oil Synthesis of Cyperus esculentus Tubers
Cyperus esculentus is an emerging economic crop with great development potential and high compre-hensive utilization value.The oil content of tuber can reach up to 38.70％.In order to obtain genetic information on the oil synthesis in its tubers,the experiment collected four samples of Cyperus esculentus tubers at different de-velopmental stages,and used the third-generation PacBio sequencing platform to sequence the full-length transcrip-tome of the samples.87 061 high-quality sequences was finally obtained through clustering,error correction and de-redundancy of the original data.And 84 394 ORFs,11 224 lncRNAs,31 995 transcription factors and 9 165 SSRs sites were predicted and analyzed by software.Among them,23 408 high-quality transcripts predicted to be gene sequences were screened for functional annotation analysis in blastx,NR,KOG,eggNOG,GO,KEGG,Pfam and other databases.A total of 19 287(82.39％)sequences were functionally annotated.NR database com-parison results showed that it had the most similar sequences with Elaeis guineensis(21.69％);GO function analysis divided the unigenes into 55 branches in three categories:biological processes,molecular functions and cellular components;KOG database analysis results were divided into 25 functional clusters;the KEGG annotation results annotate the genes involved in the transcriptome into 6 categories,a total of 188 metabolic pathways.The lipid metabolism pathway of Cyperus esculentus tubers was constructed according to the annotated functional gene sequences.And key genes for lipid synthesis such as FATA,GPAT,PDAT and DGAT were discovered.In this study,genetic information was obtained according to the third-generation transcriptome data of Cyperus esculentus tuber.At the same time,the fatty acid anabolism pathway was constructed and the full-length sequence of some functional genes were obtained,providing data support for subsequent studies on the molecular mechanism of lipid metabolism of Cyperus esculentus.
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万方数据
