Cloning of Soybean GmSDG25 Gene and Genetic Transformation of Arabi-dopsis
GmSDG25 is the homologous gene of Arabidops is histone lysine methyltransferase gene SDG25 in soy-bean.In order to explore the function of this gene,the study cloned two GmSDG25 genes from the soybean cultivar'Xi angchun2701',separately named as GmSDG25a and GmSDG25b,which of the full-length cDNA are 3 684 bp and 3 681 bp,respectively.The overexpression vectors pFGC5941-35S∶∶GmSDG25a/GmSDG25b were constructed and transformed into the Arabidopsis mutant sdg25,respectively.The homozygous transgenic Arabidopsis plants of T3 generation were obtained and subjected to phenotype identification and molecular detection.It was found that the transgenic plants sdg25-35S∶∶GmSDG25a and sdg25-35S∶∶GmSDG25b exhibited flowering later than the sdg25 mutants and at the same time as the wild-type(Col)plants.And compared with Col,the expression of flower-ing-regulated genes FLC,FT,and SOC1 in the transgenic plants has no significant difference.These results indicat-ed that both GmSDG25a and GmSDG25b could rescue the early-flowering phenotype of Arabidopsis sdg25 mutant,and restore it to the flowering phenotype of col,suggesting that GmSDG25a and GmSDG25b genes may have simi-lar biological functions of regulating plant flowering as Arabidopsis SDG25.The results of this study will provide a theoretical reference for further revealing of the mechanism of GmSDG25 involved in the regulation of flowering time in soybean.
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