Preliminary Analysis of Function of Microtubule End Binding Protein Encoding Gene CgEB1 in Colletotrichum gloeosporioides
A microtubule end binding protein encoding gene CgEB1 was identified in Colletotrichum gloeospo-rioides.To investigate the role of CgEB1 on vegetative growth and the pathogenicity of C.gloeosporioides to the host,the knock-out mutant △CgEB1 was constructed,and the growth and pathogenicity phenotype analysis was performed.The results revealed that loss of CgEB1 sharply repressed the vegetative growth,conidiation and pathogenicity of C.gloeosporioides to rubber tree leaves.The appressorium formation rate and penetration of the onion epidermis of △CgEB1 were markedly affected.Furthermore,compared with WT,△CgEB1 was more sensitive to NaCl treatment.These results suggested that CgEB1 is an important pathogenicity factor of C.gloeosporioides.
万方数据
维普
