Cloning and Expression Analysis on QtKCO1 Gene from Qiongzhuea tu-midinoda
In order to study the important role of KCO family genes in potassium transport of Qiongzhuea tu-midinoda,the KCO gene was cloned from the 2-year-old seedlings of Qiongzhuea tumidinoda by RACE technique.The gene was named QtKCO1(GenBank number:MT078981)and its expression characteristics were analyzed.The results showed that the full length of QtKCO1 gene was 1 502 bp,and the open reading frame(ORF)of QtKCO1 was 1 041 bp,encoding 347 amino acids.QtKCO1 had five transmembrane regions and showed the highest homol-ogy of 85.88％with wild two-grain wheat(Triticum dicoccoides).expressed compositionally in all tissues and organs(bamboo shoots,roots,stems,leaves),and the expression levels were in the order of bamboo shoots＞stems＞leaves＞roots.Under high concentration KC1 stress,the expression of QtKCO1 gene increased in roots,but de-creased in stems and leaves with the increase of treatment time.Compared with the control group,QtKCO1 expres-sion was up-regulated in all tissues and organs of Qiongzhuea tumidinoda under NaCl stress,and the expression level was in the order of leaves＞stems＞roots.This study provides a theoretical basis for further analysis of the role of KCO family genes in potassium transport.
Outward rectifying K+channelQiongzhuea tumidinodaQtKCO1StressGene cloning and expression
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