Mango(Mangiifera indica L.)is a sweet and nutritious tropical fruit.In order to further understand the reasons for the formation of unique flavor in mango fruit,the volatile organic compounds in the ripening process of'Tainong No.1'fruit were detected by GC-MS.It was found that a large number of terpenoids were produced during mango ripening.High-throughput sequencing technology platform Illumina HiSeq was used to sequence the transcriptome of color turning stage mango(0 d)and ripe stage mango(4 d).A total of 9 832 differentially ex-pressed genes were found.Compared with the color-changing period,4 138 differentially expressed genes were up-regulated and 5 694 differentially expressed genes were down-regulated in the mature period.This study focused on the analysis of 108 unigenes in 6 pathways related to terpenoid biosynthesis in mango fruits,including terpenoid skeleton biosynthesis,ubiquinone and other terpenoid quinone biosynthesis,diterpene biosynthesis,monoterpene biosynthesis,limonene and pinene degradation,sesquiterpene and triterpenoid biosynthesis.Thirty-six differentially expressed genes were found in the terpenoid skeleton biosynthesis pathway.The differential expression showed that the postharvest mango fruit mainly synthesized isoprenoid precursors such as IPP and DMAPP through the MVA pathway.The results enrich the transcriptome resources of mango.These genes provide a basis for further exploration of the cloning and functional analysis of mango functional genes and the biosynthesis of mango terpenoids,and also provide a reference for mango flavor quality breeding.
'Tainong No.1'mangoTranscriptome sequencingGene function annotationTerpenoids
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