Transcriptomic Analysis of Interaction between Arabidopsis thaliana and NSs Protein of Tomato Spotted Wilt Tospovirus
In order to study the role of NSs protein in the interaction mechanism between tomato spotted wilt to-spovirus and plants,this study took transgenic Arabidopsis thaliana expressing NSs gene and wild-type Arabidopsis thaliana as experimental materials,and used the Illumina RNA-seq sequencing platform for transcriptome sequ-encing,and analyzed the effects of NSs on gene expression and function by bioinformatics methods.The results showed that there were 1 258 differentially expressed genes(DEGs)between transgenic and wild-type Arabidopsis.Among them,966 DEGs were enriched in Gene Ontology(GO).Their functions were mainly referred to cell com-ponents,metabolic processes and enzyme activities.517 DEGs were enriched in KEGG pathway,it was showed that NSs reduced the expression of genes in the pathways such as phenylpropanoid biosynthesis,plant hormone signal transduction,and plant-pathogen interaction.These pathways are all related to the defense of plants against pathogenic microorganisms.101 DEGs were identified as transcription factors,belonging to 39 transcription factor families,including the MYB family that regulates phenylpropanoid biosynthesis and the AUX/IAA transcription factor family that regulates auxin signal transduction.Through the study,it is found that the interaction between NSs protein and Arabidopsis is a complicated process.NSs influences the secondary metabolism,hormone regula-tion,defense response and other biological functions of Arabidopsis.This lays the foundation for the in-depth study of the role of NSs in the interaction between tomato spotted wilt tospovirus and host plants and the control of tomato spotted wilt tospovirus.
万方数据
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