SRAP Analysis of Genetic Differences Among Superior Clones of Main Cultivated Camellia oleifera in Fujian Province
Camellia oleifera is an important wooden oil tree species in China,which has high value.This study used SRAP molecular markers to analysis the genetic diversity of 10 clones.The results showed that 12 pairs of primers were screened from 256 for PCR amplification.A total of 186 markers were obtained,including 166 poly-morphic markers,and the polymorphism ratio of 89.2％.The polymorphism of EM-2/ME-4 primer combination was up to 100％.The minimum and maximum polymorphism information values were 0.280 and 0.394,respec-tively,and the average polymorphism information value was 0.330.The minimum and maximum genetic similarity coefficients of the 10 superior clones were 0.591 4 and 0.774 2,respectively,and the average genetic similarity co-efficients were 0.670 8.Cluster analysis showed that when the genetic similar coefficient was 0.677 5,10 clones could be drawn to four categories.Min43 and Min46 gathered to the class Ⅰ,Min48,Min49,and Min53 gathered to Class Ⅱ,Min50 gathered to class Ⅲ,and Min54,Min56,Min60 and Min62 gathered to Class Ⅳ.There was a high level of genetic diversity among these 10 superior clones,and the results provided a reference for the development of hybrid breeding and molecular breeding of Camellia oleifera.
万方数据
维普
