Correlation Analysis of Microspore Development and Floral Organ Morphology in Camellia perpetua
In order to quickly select anthers in the appropriate developmental stage for ex vivo culture through the external morphological characteristics of flower organs,so as to carry out haploid breeding research,this study used the methods of Karbo magenta and DAPI fluorescence staining to observe the cytological changes of the te-trad stage,early and mid-uninucleate,late uninucleate and tricellular pollen of Camellia perpetua,and analyzed and compared the external morphological characteristics of the corresponding flower organs,which determined the op-timal buds selection period for ex vivo culture of anthers.The results showed that the microspore at different devel-opment stage of Camellia perpetua had obvious cytological characteristics,and their changes of cytological charac-teristics were closely related to the morphological characteristics of flower organs:the longitudinal diameter of flower buds and anther length had significant difference in each development stage of microspore,the color of an-ther changed significantly from white,milky-white,light yellow,yellow,to orange.So the longitudinal diameter of flower buds,anther length and anther colors could be used as external morphologic indexes to identify microspore development stage.In the study the cell nucleus of Camellia perpetua at late uninucleate microspore stage was near the pollen germination pore,and at this time the morphological characteristics of flower organs were:the longitudi-nal diameter of the flower buds was 10.48～10.56 mm,the anther length was 0.172～0.176 mm,and the anther was yellow.According to the morphological characteristics of flower organs in this period,appropriate flower buds could be selected to carry out anther ex vivo culture research,which provides the basis for the successful breeding of haploid plants.
万方数据
维普
