Bioinformatics and Expression Analysis of pAMT in Shuanla and Kunming Wrinkled Pepper
In order to analyze the differences between Shuanla(Capsicum chinense)and Kunming wrinkled pepper(Capsicum annuum)pAMT enzymes and explore the changes of pAMT expression.The pAMT gene and protein char-acteristics were studied based on gene cloning and bioinformatics methods.The relative expression levels of pAMT were determined by qPCR.Shuanla and Kunming wrinkled pepper pAMT nucleotide sequences of 1 380 bp were ob-tained with ORF tools,which encoded 459 amino acids respectively.Compared with Kunming wrinkled pepper,Shuanla pAMT had 7 synonymic mutations and 5 sites of missense mutation.The cis-element prediction results illus-trated that pepper pAMT gene might be affected by environmental and plant hormones.Be predicted pAMT protein belonged to Ⅰ type folding,Ⅲ transaminase,as fat-soluble,hydrophilicity of unstable protein,mainly engaged in the cytoplasm,no signal peptide and the membrane structure.It was different for the ligand binding sites and active sites of pAMT.AMT of various species is evolutionarily conservative,but there had some differences among subgroups.The pAMT genes expressed that first increased and then sharply decreased under different development periods.It was higher for pAMT genes expression in the open field than greenhouses.Under the same environmental conditions,pAMT gene expression of Shuanla overall higher than Kunming wrinkled pepper.Different exogenous substances af-fect the expression of pAMT in a certain period of time.Compared with Kunming wrinkled pepper,changes in the pAMT gene and protein of Shuanla may be the reason for its high hotness.Capsaicin biosynthesis by regulating the ex-pression of pAMT in pepper under different growth environment,fruit development stages and exogenous substances.
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