Cloning of Rice NAC073 Gene and Construction of Overexpression Vector
Plant transcription factors play a crucial role in regulating growth and development as well as respond-ing to environmental stresses.In order to study the function and mechanism of NAC transcription factors in rice re-sponse to stress,especially salt stress,the rice strain 58M which independently selected by our laboratory was used as the test material.On the basis of transcriptome sequencing,a NAC family transcription factor OsNAC073 was obtained by extracting RNA and reverse transcription to obtain cDNA clone.Bioinformatics analysis was carried out,and the overexpression vector was constructed by homologous recombination.The results of the study indi-cate,the total length of the cDNA of OsNAC073 is 942 bp,encoding 313 amino acids,the relative molecular weight of the protein is 34.029 33 kD,the theoretical isoelectric point is 9.18,the total average hydrophilicity in-dex is-0.534,and the aliphatic index is 63.04.The gene has a highly conserved NAM domain and belongs to the NAC family.The protein has no transmembrane properties and signal peptide,and is a non-secretory protein.The secondary structure consists of 49.52％random coil and 25.56％α-helix,18.21％extended strand,6.71％β-turn composition.The genetic relationship shows that it has the highest homology with Bamb us a emeiensis.The over-expression vector pC1302-35S:NAC073-GFP was successfully constructed in this experiment.It provide theoreti-cal basis for the follow-up transgenic experiment to explore the function of rice NAC transcription factor.
NETL
NSTL
万方数据
