Molecular Detection of TaSus2-2B and TaGw8-Bl Genes Controlling Grain Weight in Huanghuai Southern Wheat Region
In order to understand the distribution of allelic variants of wheat kernel weight-related genes TaSus2-2B and TaGw8-B1 in newly bread wheat varieties from Huanghuai southern region,in this study,the functional markers TaSus2-1,TaSus2-2 and TaGw8-7B were used to detect the alleles of TaSus2-2B and TaGw8-B1 in 94 test materials,and explored the relationship between the alleles and grain weight.The results showed that the aver-age value of thousand-grain weight was 45.46 g,the standard deviation was 2.98,and the range was 39.5～52.3 g.The medium grain weight of the tested materials accounted for a higher proportion.In the TaSus2-2B locus,the fre-quency of the tested wheat varieties with TaSus2-2Ba allele was 25.53％,and TaSus2-2Bb allele was 74.47％.The grain weight of the TaSus2-2Ba allele was significantly higher than that of TaSus2-2Bb allele(P＜0.05),TaSus2-2Ba allele was associated with high grain weight,and TaSus2-2Bb allele was associated with low grain weight.In the TaGw8-B1 locus,only TaGw8-B1a alleles were detected,and the polymorphism of TaGw8-B1 locus in these materials was relatively single.This study can provide reference information for the molecular improvement of wheat grain weight in Huanghuai southern wheat region.
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