Construction of Expression Vector and Expression Pattern Analysis of Soybean sHSP Gene Glyma.07G141700.1
In order to study the function of soybean small heat shock proteins related gene Glyma.07G141700.1,this study constructed the Glyma.07G141700.1 plant expression vector.The RT-qPCR method detected the relative expression of Glyma.07G141700.1 gene in different tissues and 250 mmol/L NaCl stress for 0,2,6,12,24 h in soy-bean roots.The results showed that the expression of Glyma.07G1417001.1 was highest in stem.The expression level of Glyma.07G141700.1 was the highest at 2 h and the lowest at 12 h under 250 mmol/L NaCl stress.The analysis of Glyma.07G141700.1 using bioinformatics methods showed that the coding region of Glyma.07G141700.1 is 471 bp long and encodes 156 amino acids.The functional domain is predicted to have a small heat shock protein sHSP domain,located at the 36～153th amino acid.The results of multiple sequence alignment and phylogenetic tree analysis show that the target gene is closely related to wild soybean(KHN05131.1,Glycine soja),soybean(XP_003552209.1,Glycine max),soybean(KAG4922021.1,Glycine max).This experiment provides a ba-sis for further studying the biological functions of soybean sHSPs gene Glyma.07G141700.1.
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