In order to study the function of soybean small heat shock proteins related gene Glyma.07G141700.1,this study constructed the Glyma.07G141700.1 plant expression vector.The RT-qPCR method detected the relative expression of Glyma.07G141700.1 gene in different tissues and 250 mmol/L NaCl stress for 0,2,6,12,24 h in soy-bean roots.The results showed that the expression of Glyma.07G1417001.1 was highest in stem.The expression level of Glyma.07G141700.1 was the highest at 2 h and the lowest at 12 h under 250 mmol/L NaCl stress.The analysis of Glyma.07G141700.1 using bioinformatics methods showed that the coding region of Glyma.07G141700.1 is 471 bp long and encodes 156 amino acids.The functional domain is predicted to have a small heat shock protein sHSP domain,located at the 36～153th amino acid.The results of multiple sequence alignment and phylogenetic tree analysis show that the target gene is closely related to wild soybean(KHN05131.1,Glycine soja),soybean(XP_003552209.1,Glycine max),soybean(KAG4922021.1,Glycine max).This experiment provides a ba-sis for further studying the biological functions of soybean sHSPs gene Glyma.07G141700.1.
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