Cloning and Expression Analysis of PteAUX1 Gene Related to Bulbil Development in Pinellia ternata
Auxin may play an important regulatory role in bulbil development of Pinellia ternata(Thunb.)Breit.,but the molecular mechanism of auxin regulating the development of the bulbil is still unclear.In this study,RNA-seq results of P.ternata and P.cordata,which are relatives,were used to screen candidate unigenes that are involved in regulation of auxin concentration during bulbil development.Full CDS sequences were obtained by RACE cloning or were confirmed by sequencing.Furthermore,quantitative real-time PCR was used to analyze the spatio-temporal expression model of candidate genes during bulbil development of P.ternata.The results showed that there were differentially expressed genes in auxin synthesis,inactivation and transport during bud formation,and members of the AUX1/LAX family of auxin input proteins(PteAUX1 and PcoLAX2)were differentially expr-essed in the two species during bud formation;sequence analysis showed that the secondary structure of encoded proteins had 11 transmembrane domains,and functionally related important sites were conserved;PteAUX1 is differentially expressed on the upper/lower parts of P.ternata petiole with the occurrence of bead buds.These results indicated that differential expression of auxin influx carrier AUX1/LAX family members in the upper/lower regions of P.ternata petioles during different stages of bulbil formation may lead to localized accumulation of endogenous auxin,thereby regulating bulbil initiation.The research results can provide a theoretical basis for further exploring the signal transduction pathways of bulbil development regulation of P.ternata.
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