Identification of Drought Resistance and Difference Analysis of Related Gene Loci of Japonica Rice in Yunnan Plateau
To explore the resources of high-quality drought resistant varieties and clarify the genetic variation a-mong drought resistant related gene loci.In this study,198 japonica rice variety resources in Yunnan,China and abroad were used as materials.Through field drought resistance identification,cluster analysis and phenotypic variation analysis,excellent drought resistant japonica rice variety resources were selected,and the differential loci of drought related genes were analyzed by rice 56K SNP chip.Cluster analysis showed the materials were divided into five groups,Yunnan conventional japonica rice series,Yunnan Dian-type japonica hybrid rice series and japonica rice resources from China and abroad were divided into different groups,showing different drought resis-tance.The phenotype variation coefficient ranged from 12.05%to 94.08%,among which the variation coefficient of panicle extension length was the largest and the panicle length was the smallest.The angle of flag leaf,panicle length,flag leaf length and plant height decreased with the increase of altitude.Five extremely drought resistant va-rieties,six medium drought resistant varieties and ten general drought resistant varieties were comprehensively e-valuated.The overall drought resistance of Dian-type japonica hybrid rice variety series was the best.56K SNP chip detected 114 drought related genes,including 357 loci,66.03%of the site variation in CDS region belongs to missense mutation.The high-quality drought resistant resources and abundant variation loci obtained in this study can provide effective support for drought resistant molecular breeding of rice in the future.