首页|云南高原粳稻抗旱性鉴定及相关基因位点差异分析

云南高原粳稻抗旱性鉴定及相关基因位点差异分析

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为了挖掘优质抗旱品种资源,明确抗旱相关基因位点间的遗传变异.本研究以198份国内外的粳稻品种资源为材料,进行田间抗旱鉴定,通过聚类分析和表型变异分析,筛选出优良抗旱粳稻品种资源,利用水稻56K SNP芯片,解析抗旱相关基因的差异位点.聚类分析表明,供试材料被聚成为5类,云南常规粳稻系列、滇型杂交粳稻系列和国内外粳稻资源划分为不同类群,表现出不同的抗旱性.表型变异系数变幅在12.05%~94.08%之间,其中穗伸出度的变异系数最大,穗长最小.剑叶角度、穗长、剑叶长度和株高都表现出随海拔高度的增加而逐渐减少的趋势.综合评价出5个极端抗旱品种,6个中等抗旱品种,10个一般抗旱品种,滇粳杂交稻系列抗旱性总体表现较好.56KSNP芯片检测到抗旱相关基因114个,包含357个位点,CDS区域中66.03%的位点变异属于错义突变.本研究获得的优质抗旱品种资源和丰富的变异位点可为今后水稻的抗旱分子育种提供理论依据.
Identification of Drought Resistance and Difference Analysis of Related Gene Loci of Japonica Rice in Yunnan Plateau
To explore the resources of high-quality drought resistant varieties and clarify the genetic variation a-mong drought resistant related gene loci.In this study,198 japonica rice variety resources in Yunnan,China and abroad were used as materials.Through field drought resistance identification,cluster analysis and phenotypic variation analysis,excellent drought resistant japonica rice variety resources were selected,and the differential loci of drought related genes were analyzed by rice 56K SNP chip.Cluster analysis showed the materials were divided into five groups,Yunnan conventional japonica rice series,Yunnan Dian-type japonica hybrid rice series and japonica rice resources from China and abroad were divided into different groups,showing different drought resis-tance.The phenotype variation coefficient ranged from 12.05%to 94.08%,among which the variation coefficient of panicle extension length was the largest and the panicle length was the smallest.The angle of flag leaf,panicle length,flag leaf length and plant height decreased with the increase of altitude.Five extremely drought resistant va-rieties,six medium drought resistant varieties and ten general drought resistant varieties were comprehensively e-valuated.The overall drought resistance of Dian-type japonica hybrid rice variety series was the best.56K SNP chip detected 114 drought related genes,including 357 loci,66.03%of the site variation in CDS region belongs to missense mutation.The high-quality drought resistant resources and abundant variation loci obtained in this study can provide effective support for drought resistant molecular breeding of rice in the future.

Japonica riceDrought resistanceSNP lociGermplasm resources

罗迪、王天杰、李娟、朱骞、何玉、李俊杰、蒋春和、黄大军、陈丽娟、李东宣

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云南农业大学稻作研究所,昆明,650201

云南农业大学,云南省作物生产与智慧农业重点实验室,昆明,650201

云南农业大学,省部共建云南生物资源保护与利用国家重点实验室,昆明,650201

粳稻 抗旱 SNP位点 种质资源

2024

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2024.22(18)