Analysis of Genetic Diversity of Rosa roxburghii Tratt.Based on ITS Se-quence
In order to conserve and manage Rosa roxburghii Tratt.germplasm,the genetic diversity and popula-tion genetic structure of 255 individuals from 29 R.roxburghii populations were analyzed by ITS sequence.The re-sults showed that:the length of ITS fragment alignment of 255 individuals was 697 bp,G+C was 57.8％.Nine nu-cleotide mutation sites(1.36％)were detected,and 10 haplotypes were defined.Low genetic diversity levels were found in 225 wild pear samples(Hd=0.2470,π=0.000 40),and the nucleotide polymorphism levels were significantly different among different populations(Hd=0～0.666 7,π=0～0.001 02).The genetic variation was 85.69％,the genetic differentiation Fst was 0.143,and the average Nem gene flow was 2.994.There was a significant correlation be tween genetic and geographic distance between populations(r=0.415,P=0.010).The results suggest that populations with larger populations and higher ITS haplotype diversity(such as SC)should adopt the strategy of local preserva-tion,and populations with unique haplotypes(such as FQ)should be protected first.This study provides a theoreti-cal basis for the protection of wild R.roxburghii resources.
Rosa roxburghiiITS sequenceGenetic diversityGenetic structureGermplasm conservation
NETL
NSTL
万方数据
