Phylogeographic Structure of Cryptomeria japonica in East Asia Using Chloroplast Microsatellite Markers
Cryptomeria japonica is an endemic relic plant in East Asia,which has two recognized varieties of C.japonica var.sinensis and C.japonica var.japonica.The lineage spatial pattern and the formation mechanism of this species are still unclear.In this study,we used chloroplast microsatellite(cpSSR)markers to explore the genet-ic diversity and phylogeographic structure of C.japonica populations and to analyze the relationship between spa-tial distribution and phylogenetic evolution.The results showed that a total of 127 haplotypes was detected based on 11 polymorphic cpSSR loci,and the number of haplotypes(A)ranged from 4 to 16,among which the num-ber of private haplotypes(P)ranged from 1 to 11 in each population.In the species level,C.japonica has high ge-netic diversity(H=0.890)and significant genetic differentiation(FST=0.594,P＜0.001).Consistent with its spatial distribution,all populations can be divided into two groups of C.japonica var.sinensis and C.japonica var.japon-ica based on cpSSR data.There was an obvious phylogeographic structure that existed among populations(Gsr=0.102＜Rsr=0.702)in East Asia.Combined with the climate change data,it is speculated that the Quaternary cli-mate change and long-term geographical isolation were the main factors for the phylogeographic distribution pat-tern of different lineages in East Asia.The results can provide scientific basis for the flora evolution of East Asia and the conservation of genetic resources of C.japonica.
NETL
NSTL
万方数据
