Communities Structure of Soil Bacterial of Rhizosphere of Broccoli in Coastal Reclamation Areas Vary with Reclamation Duration
In order to reveal the changes of soil quality after reclamation and provide a theoretical basis for the scientific regulation of the soil ecosystem,the changes of bacterial community structure in rhizosphere soil of broc-coli collected from coastal reclamation area with different years of reclamation(13 a,36 a,57 a)in Taizhou estu-ary were analyzed by using the Illumina Miseq high-throughput sequencing technology.The results showed that the total of 126 355.333 effective sequence readings and 7 660 OTUs were obtained from nine soil samples based on high-throughput sequencing of the 16S gene.Bacterial species detected in these samples covered 23 phylum,82 class,148 orders,276 families,651 gene.The abundance and diversity of bacterial communities in soil rhizosphere of broccoli collected from coastal reclamation areas with 36 years of reclamation was the highest among all soil samples,followed by soil samples of 57 years of reclamation,while that of 13 years of reclamation was the lowest among all soil samples.The dominant phyla were Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria and Cyanobacteria in all soil samples.At the class level,Alphaproteobacteria,Gammaproteobacteria,Betaproteobacte-ria,Actinobacteria,Acidobacteria and Gemmatimonadetes were the predominant bacterial communities existed in all the soil samples.The long term land reclamation had significant effects on soil bacteria communities composi-tions.With increasing of reclamation,relative abundances of Proteobacteria,Acidobacteria,Actinobacteria initial-ly increased and then decreased,while the relative abundance of Cyanobacteria was characterized by increase trend and that of Gemmatimonadetes was characterized by decrease trend.
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