Alternative Splicing of Rice OsEnS51 and Structural Analysis of Encoding Protein
In this study,bioinformatics,RT-PCR and TA cloning techniques were used to identify alternatively spliced transcripts of rice endosperm-specific expression gene OsEnS51,and the expression of the spliceosome were then analyzed.4 potential alternatively spliced transcripts were predicted with bioinformatics analysis.Of these tran-scripts,OsEnS51.1 and OsEnS51.2 contains 2 Cupin_1 domains,while OsEnS51.3 and OsEnS51.4 only contains 1 Cupin_1 domain respectively,suggesting structural differentiation of various OsEnS51 transcripts.According to the sequence information of different alternatively spliced transcripts,specific primers were designed for further RT-PCR amplification.Combined with TA cloning and sequencing,three of them were successfully identified alterna-tively spliced transcripts,designated OsEnS51.1,OsEnS51.3 and OsEnS51.4.OsEnS51.1~OsEnS51.4 was high ex-pressed in seeds 7~15 days following pollination,whose transcription products continued to accumulate with the de-velopment of seeds.Higher expression level of OsEnS51.4 was detected compared to that of OsEnS51.1,indicating divergent expression patterns of different alternatively spliced transcripts of OsEnS51.Altogether,OsEnS51 has at least 3 alternative splicing transcripts with differentiated encoding protein structures.These results laid the founda-tion for further research on the biological functions of OsEnS51.
Oryza sativaSeed-specifically expressed genesAlternative splicingExpression analysisThree-dimensional protein structure