不同大麦品种B-醇溶蛋白基因的克隆和序列分析
Cloning and Sequence Analysis of B-hordein Genes from Different Barley Varieties
赵永英 1赵献林 1相志国 1张丹 1杨红珊 1张玉杨 1王美芳1
作者信息
- 1. 河南省农业科学院小麦研究所,郑州,450002
- 折叠
摘要
大麦醇溶蛋白是影响大麦食品加工、麦芽制备和饲用价值的主要因素之一.B-醇溶蛋白占总醇溶蛋白的70％～80％,对品质的贡献相对较大.本研究对来自不同国家和地区的18份大麦材料的B-醇溶蛋白基因进行了克隆和鉴定,共得到27个完整的基因序列,包括22个编码基因和5个假基因.经与GenBank注册的序列进行比对,它们之间的碱基相似度为79.6％～99.5％,说明这27个基因序列全部是新基因或等位变异.22个基因的推断氨基酸序列表明,其中18个基因含有8个保守的半胱氨酸残基,2个基因含有7个半胱氨酸,其余2个则含有9个半胱氨酸,这在大麦中是首次发现,其可能属于编码优质蛋白亚基的基因.研究结果将为进一步研究大麦醇溶基因的特征特性以及培育优质大麦品种提供理论参考.
Abstract
Barley prolamins is one of the main factors affecting the food processing,malt production and feeding value.B-hordeins account for 70％～80％of whole prolamins and have great effects on barley quality.18 barley va-rieties from different countries and areas were used to clone the B-hordein genes,from which 27 complete gene se-quences were cloned,including 22 coding genes and 5 pseudogenes.By comparing with the sequences registered by GenBank,the base similarity between them was 79.6％～99.5％,indicating that all the 27 gene sequences were new genes or alleles.The deduced amino-acid sequences of 22 genes showed that 18 had a typical of 8 conserved cysteine(Cys)residues,2 genes contained 7 cysteine,and the remaining 2 genes contained 9 cysteine.The latter four genes were found the first time in barley,and might be the genes to code the good-quality subunits.The results above may provide a theoretical reference for further studying the characterization of B-hordein genes and breed-ing the barley varieties with good qualities.
关键词
大麦/B-醇溶蛋白/基因/克隆/序列分析Key words
Barley/B-hordeins/Gene/Cloning/Sequence analysis引用本文复制引用
基金项目
河南省农业科学院新兴学科项目(2022XK02)
河南省农业科学院新兴学科项目(2021XK07)
河南省科技攻关项目(222102110428)
河南省农业科学院自主创新项目(2021ZC02)
出版年
2024
NETL
NSTL
万方数据