Isolation and Expression Analysis of Broccoli Transcription Factor Gene BoiWRKY7
WRKY transcription factors play an important role in plant stress defense,and they participate in the regulation of biotic and abiotic responses.In the study,the aim is to isolate a WRKY transcription factor,namely BoiWRKY7,from Brassica oleracea var.italic a,and to obtain its expression patterns challenged by Xanthomonas campestris pv.campestris(Xcc)and Hyaloperonospora parasitica.The results showed that the full genomic DNA of BoiWRKY7 was 1 416 bp,containing two introns of 94 and 308 bp in length;the complete coding sequence was 1 014 bp,encoding 377 amino acids;phylogenetic analysis indicated that BoiWRKY7 was grouped with WRKY7 from B.oleracea,with a support rate of 100％;expression analysis revealed that the expression of BoiWRKY7 was induced by Xcc,and the highest expression levels were observed at 24 and 48 h after pathogen inoculation.Additionally,BoiWRKY7 was induced by H.parasitica,reaching the highest level at 48 h.Isolation and expression analysis of BoiWRKY7 laid a basis for future investigation on functional identification of broccoli disease resistance responses.
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