WRKY transcription factors play an important role in plant stress defense,and they participate in the regulation of biotic and abiotic responses.In the study,the aim is to isolate a WRKY transcription factor,namely BoiWRKY7,from Brassica oleracea var.italic a,and to obtain its expression patterns challenged by Xanthomonas campestris pv.campestris(Xcc)and Hyaloperonospora parasitica.The results showed that the full genomic DNA of BoiWRKY7 was 1 416 bp,containing two introns of 94 and 308 bp in length;the complete coding sequence was 1 014 bp,encoding 377 amino acids;phylogenetic analysis indicated that BoiWRKY7 was grouped with WRKY7 from B.oleracea,with a support rate of 100％;expression analysis revealed that the expression of BoiWRKY7 was induced by Xcc,and the highest expression levels were observed at 24 and 48 h after pathogen inoculation.Additionally,BoiWRKY7 was induced by H.parasitica,reaching the highest level at 48 h.Isolation and expression analysis of BoiWRKY7 laid a basis for future investigation on functional identification of broccoli disease resistance responses.
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