青花菜转录因子基因BoiWRKY7的克隆及表达分析
Isolation and Expression Analysis of Broccoli Transcription Factor Gene BoiWRKY7
朱晏 1蒋明 1张慧娟 1黄雯馨 1吴倩 1周梦亚 1张胜2
作者信息
- 1. 台州学院生命科学学院,台州,318000
- 2. 台州市农业技术推广中心,台州,318000
- 折叠
摘要
WRKY转录因子在植物逆境防御中起着重要作用,它们参与生物胁迫与非生物胁迫的调控.本研究以青花菜为材料,在克隆转录因子基因BoiWRKY7的基础上,开展序列分析和系统发育分析,并明确其在野油菜黄单胞菌(Xanthomonas campestris pv.campestris)和霜霉菌(Hyaloperonospora parasitica)侵染下的表达模式.结果表明,BoiWRKY7的DNA全长为1 416 bp,具2个内含子,长度分别为94和308 bp;基因的编码区全长为1 014 bp,编码337个氨基酸;系统发育分析结果表明,BoiWRKY7与野甘蓝WRKY7聚为一组,支持率达100％;基因表达分析结果表明,BoiWRKY7的表达受野油菜黄单胞菌的诱导,表达水平在24和48 h时的表达量最大;BoiWRKY7的表达还受霜霉菌的诱导,在48 h时的表达量最大.青花菜BoiWRKY7的克隆与表达研究,为将来开展该基因的功能鉴定提供了基础.
Abstract
WRKY transcription factors play an important role in plant stress defense,and they participate in the regulation of biotic and abiotic responses.In the study,the aim is to isolate a WRKY transcription factor,namely BoiWRKY7,from Brassica oleracea var.italic a,and to obtain its expression patterns challenged by Xanthomonas campestris pv.campestris(Xcc)and Hyaloperonospora parasitica.The results showed that the full genomic DNA of BoiWRKY7 was 1 416 bp,containing two introns of 94 and 308 bp in length;the complete coding sequence was 1 014 bp,encoding 377 amino acids;phylogenetic analysis indicated that BoiWRKY7 was grouped with WRKY7 from B.oleracea,with a support rate of 100％;expression analysis revealed that the expression of BoiWRKY7 was induced by Xcc,and the highest expression levels were observed at 24 and 48 h after pathogen inoculation.Additionally,BoiWRKY7 was induced by H.parasitica,reaching the highest level at 48 h.Isolation and expression analysis of BoiWRKY7 laid a basis for future investigation on functional identification of broccoli disease resistance responses.
关键词
WRKY/青花菜/黑腐病/霜霉病/表达Key words
WRKY/Broccoli/Black rot/Downy mildew/Expression引用本文复制引用
基金项目
台州市科技计划项目(1901ny08)
浙江省自然科学基金项目(LY19C150004)
出版年
2024
NETL
NSTL
万方数据