Cloning and Spatiotemporal Expression Characteristics of Class-A MADS-box Gene APETALA1-like in Cymbidium sinense
In this research,the APETALA1-like(AP1)gene of Cymbidium sinense was cloned,analyzed and ex-pressed by real-time fluorescence quantitative analysis(RT-qPCR).The results showed that the cloned AP1 was MIKC MADS-box gene with conserved MADS region and relatively conserved K region,and had the highest ho-mology(100％)with Phalaenopsis hybrid(AFD36438.1).The gene was named as CsAP1,and the accession num-ber was MW654192.RT-qPCR analysis showed that CsAP1 was expressed in tissues of Cymbidium sinense in dif ferent periods,and the order of relative expression abundance from high to low was calyx(Ⅲ)＞leaf(Ⅲ)＞root(Ⅰ)＞lip petal(Ⅲ)＞inflorescence rachis(Ⅲ)＞leaf(Ⅱ)＞root(Ⅲ)＞root(Ⅱ)＞inflorescence rachis(Ⅱ)＞petal(Ⅲ)＞leaf(Ⅰ)＞bud(Ⅱ)＞ovary(Ⅲ)＞gynostemium(Ⅲ).CsAP1 was highly expressed in calyx and leaves at full-blossom stage,which was involved in the formation of sepals of flower organs and might regulate flowering time.In terms of growth and development period,the expression level at full-blossom stage was the highest,which indicated that CsAP1 regulated flowering time.With respect to leaves,the expression level increased gradually in three stages,which suggested that CsAP1 promoted flowering.For flowers,the expression level of CsAP1 gradually decreased from calyx to petal and then to gynostemium.This research provides a basis for further studying the function and molecular mechanism of AP1 gene in Cymbidium sinense.
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