Cloning and Prokaryotic Expression of Citrus Chitinase Cs1g26330.1 Gene
Chitinase has glycoside hydrolase and lysozyme activities,can degrades chitin,and is an important fun-gal disease resistance gene in plants.In order to study the catalytic function of chitinases in citru and understand the properties of the proteins,and lay a foundation for the subsequent research on disease resistance breeding.In this study,the full-length coding region sequence of chitinase gene Cs1g26330.1 was amplified from a cDNA li-brary of Citrus Shatangju,then cloned into the pEASY-Blunt E1 vector,after sequenced and verified,the recombi-nant plasmid was transformed E.coli BL21(DE3)compendent cells,the gene was expression successfully induced by IPTG,and the recombinant protein was detected by SDS-PAGE electrophoresis.The chitinase expression in E.coli obtained in this study can be used as a protein antigen to develop specific antibodies,which can be used for further study the function of Citrus chitinase,and is also expected to be used as an efficient and non-toxic pesticide for plant disease resistance,or to produce degradative enzymes for utilization of chitin.
CitrusChitinChitinaseProkaryotic expressionRecombinant protein
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