F3'H Gene Cloning and Expression Analysis of Original Plants of Dragon's Blood
Flavonoid 3'-hydroxylase(F3'H)is one of the key enzymes in plant flavonoid metabolism,and it is invo-lved in the biosynthesis of dragon's blood in Dracaena trees.In order to reveal the F3'H gene sequence and expres-sion pattern of Dracaena trees,the F3'H gene sequences of three Dracaena(Dracaena cochinchine ns is,Dracaena cambodiana,and Aizong)were cloned and bioinformatics analysis was conducted in this study.RT-qPCR was used to analyze the expression levels of the F3'H gene in different tissues.The result showed that a 1 533 bp sequence of F3'H gene encoding 510 amino acid residues was obtained from all three Dracaena species.Bioinformatics analy-sis showed that their sequence homology reached 99.57％,and the physicochemical properties,secondary structure and tertiary structure of amino acid sequences were almost identical.Phylogenetic analysis showed that the F3'H genes of Dracaena trees were clustered into one branch.Interestingly,the F3'H genes of genus Dracaena does not clus-ter with the Liliaceae.In addition,the F3'H genes of monocotyledons and dicotyledons were not clustered separate-ly in phylogenetic tree.RT-qPCR analysis showed that the expression level of F3'H in stem of Dra-caena cochinchinensis was much higher than that in root and leaf.However,the expression level of F3'H in root and leaf was higher than that in stem for Dracaena cambodiana and Aizong(Dracaena sp.).This study would be helpful to reveal the potential role of F3'H in dragon's blood biosynthesis of Dracaena and provide a molecular basis for the development of artificial lipid formation technology of dragon's blood.
NETL
NSTL
万方数据
