Comparison of Genomic DNA Extraction Methods from Cynanchum bungei Decne.
For the purpose of exploring the most pertinent method of genomic DNA extraction from Cynanchum bungei,this study utilized the from fresh foliage of C.bungei the raw material,and gleaned the genomic DNA of C.bungei with five distinct extraction methods.Furthermore,the DNA was examined by ultra-micro spectrophotome-ter,gel electrophoresis,and polymerase chain reaction(PCR)testing to assessed and down-select the most appli-cable method for C.bungei DNA extraction.Among all the methods,CTAB method and improved CTAB method were proved to be suitable for genome extraction of this specific application,where CTAB method provided the highest concentration of DNA,with the proof of single and clear gel band result.This study provides future refer-ence for the selection of high-quality DNA extraction methods for C.bungei,and provides scientific evidence for subsequent experiments in the aspect of molecular biology.
Cynanchum bungei Decne.Genomic DNA extractionCTABImproved CTAB
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