泰山白首乌基因组DNA提取方法的比较
Comparison of Genomic DNA Extraction Methods from Cynanchum bungei Decne.
王昊冉 1盛可心 1王飞 2徐凌川1
作者信息
- 1. 山东中医药大学药学院,济南,250014
- 2. 山东御华景宸生态农业发展有限公司,临沂,273418
- 折叠
摘要
为了探寻最适合泰山白首乌新鲜叶片基因组DNA提取的方法,本研究以泰山白首乌新鲜叶片为材料,以5种不同的基因组提取方法提取泰山白首乌基因组DNA,并将提取到的基因组DNA经超微量分光光度计、凝胶电泳检测、聚合酶链式反应(PCR)检测,找出最适合提取泰山白首乌叶片基因组DNA的方法.在所有地方法中,CTAB法、改良CTAB法均可用于基因组提取,其中CTAB法提取的DNA浓度最高,凝胶条带单一清晰.本研究为泰山白首乌基因组高质量DNA的提取方法选择提供了参考,为后续进行分子生物实验提供科学依据.
Abstract
For the purpose of exploring the most pertinent method of genomic DNA extraction from Cynanchum bungei,this study utilized the from fresh foliage of C.bungei the raw material,and gleaned the genomic DNA of C.bungei with five distinct extraction methods.Furthermore,the DNA was examined by ultra-micro spectrophotome-ter,gel electrophoresis,and polymerase chain reaction(PCR)testing to assessed and down-select the most appli-cable method for C.bungei DNA extraction.Among all the methods,CTAB method and improved CTAB method were proved to be suitable for genome extraction of this specific application,where CTAB method provided the highest concentration of DNA,with the proof of single and clear gel band result.This study provides future refer-ence for the selection of high-quality DNA extraction methods for C.bungei,and provides scientific evidence for subsequent experiments in the aspect of molecular biology.
关键词
泰山白首乌(Cynanchum/bungei/Decne.)/基因组DNA提取/CTAB/改良CTABKey words
Cynanchum bungei Decne./Genomic DNA extraction/CTAB/Improved CTAB引用本文复制引用
基金项目
山东省自然科学基金项目(ZR2012HM047)
出版年
2024
NETL
NSTL
万方数据