Development of Molecular Markers for Genotyping Heat-tolerant Gene TT1 in Rice Based on KASP Technology
High temperature is one of the environmental factors that have an important impact on the yield and quality of rice,with the gradual warming of the global climate,heat stress poses a serious threat to rice production.It has reported that the OgTT1 allele in African cultivated rice CG14 can significantly improve the heat tolerance of rice.In order to use OgTT1 alleles to breed heat-tolerant rice varieties more efficiently,a high-throughput molecular marker TT1-KASP was designed based on the functional SNP sites of OgTT1 alleles.This molecular marker was used to detect two F4 generation recombinant inbred line(RIL)populations derived from the cross of NIL(CG14)x Shenhui26 and NIL(CG14)xShen6B.The results showed that among the 65 lines of RIL,4 lines did not contain OgTT1 alleles,53 strains were homozygous for OgTT1 alleles,while the remaining 8 lines were heterozygous genotypes.After sequencing the genes of 65 F4 recombinant inbred lines,it was found that the obtained gene sequences were completely consistent with the sequences obtained from the TT1-KASP marker.These results indicate that the TT1-KASP molecular marker can be used for marker-assisted selection breeding of heat-tolerant rice varieties.
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