SSR Distribution and Sequence Characteristics of the Full-length Transcrip-tome of Pteroceltis tatarinowii
In order to provide a basis for the development of SSR markers in Pterocarpus tatarinowii,SSR loci in-formation was analyzed in the full-length transcriptome of P.tatarinowii by bioinformatics method.MISA software was used to perform SSR loci search and information analysis in gene sequences obtained by the full-length tran-scriptome.A total of 6 524 SSR loci distributed were detected in 11 801 genes.The frequency of SSR loci was 55.29％,with an average distribution of 2.49 kb in length.In the mononucleotide repeat type accounting for 44.70％in all repeat types,the dominating motif was A/T accounting for 99.28％.In the dinucleotide repeat type accounting for 19.33％in all repeat types,the dominating motif was AG/TC accounting for 37.67％.Most of the SSR makers were 10～25 bp in length with the corresponding percentage 77.16％.The results showed that there were abundant types of SSR loci and high polymorphism in P.tatarinowii,which could provide a theoretical basis for the development and utilization of S SR molecular markers,analysis of genetic diversity of germplasm re-sources and molecular marker assisted breeding.
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