巴戟天离体保存与茎尖小滴玻璃化法超低温保存体系的构建
Construction of in Vitro Preservation and Shoot Tips Droplet-vitrification Cryopreservation System of Morinda officinalis How
颜航 1李春燕 2李志英 3王敏瑞 3荆永琳 3王小冰 3李玉莲 3陈浪欣 3徐立3
作者信息
- 1. 长江大学农学院,荆州,434025;中国热带农业科学院热带作物品种资源研究所,农业农村部中药材生物学与栽培重点实验室,海南省热带作物资源遗传改良与创新重点实验室,儋州,571737
- 2. 中国热带农业科学院热带作物品种资源研究所,农业农村部中药材生物学与栽培重点实验室,海南省热带作物资源遗传改良与创新重点实验室,儋州,571737;海南大学园艺学院,海口,570228
- 3. 中国热带农业科学院热带作物品种资源研究所,农业农村部中药材生物学与栽培重点实验室,海南省热带作物资源遗传改良与创新重点实验室,儋州,571737
- 折叠
摘要
本研究以巴戟天为试验材料,探究试管苗生长的最适培养容器及离体保存的最适培养基,并以此为基础构建茎尖小滴玻璃化法超低温保存体系.结果表明,组培袋中植株长势更好且延缓生长方面较玻璃瓶和塑料瓶差异不显著,可作为巴戟天离体保存培养容器.高糖低激素培养基在巴戟天离体保存方面有显著效果,试管苗的保存时间从4个月延长至6个月.以高糖低激素培养基预处理的植株茎尖经过常温玻璃法小滴玻璃化法超低温保存后再生率可达13.3％.本研究结果为巴戟天离体保存和其茎尖小玻璃化超低温保存体系的建立奠定基础.
Abstract
In this study,the Morinda officinalis was used as the experimental material to explore the optimal cul-ture container for the growth of test-tube seedlings and the optimal medium for in vitro preservation.Based on this,the cryopreservation system of shoot tips droplet-vitrification was constructed.The results showed that the plant growth in the tissue culture bag was better and the growth delay was not significantly different from that in the glass bottle and the plastic bottle,which could be used as the in vitro preservation culture container of Morinda officinalis.The high sugar and low hormone medium has a significant effect on the preservation of Morinda officinalis in vitro.The preservation time of tissue culture seedlings was extended from 4 months to 6 months.The regeneration rate of shoot tips pretreated with high sugar and low hormone medium could reach 13.3％after cryopreservation by room temperature droplet-vitrification.The research results laid a foundation for the in vitro preservation of Morinda officinalis and for establishing its shoot tips droplet-vitrification cryopreservation system.
关键词
巴戟天/组培袋/高糖低激素培养/离体保存/小滴玻璃化法/超低温保存Key words
Morinda officinalis/Tissue culture bag/High sugar and low hormone culture/In vitro conservation/Droplet-vitrification/Cryopreservation引用本文复制引用
基金项目
国家重点研发计划项目(2021YFD1200204)
中央级公益性科研院所基本科研业务费项目(1630032022010)
农业种质资源普查收集保护鉴定服务项目(19221892)
出版年
2024
NETL
NSTL
万方数据