Iris is an important ornamental flower,which can be widely planted in water area,dry land and other environments,with high economic value,ornamental value and medicinal value.Iris mostly propagated by divid-ing plants and sowing seeds,which has low reproductive efficiency.The regeneration system established by in vitro propagation can rapidly expand the reproductive coefficient and provides an inevitable trend to meet the market development needs.In this study,the studies on the selection of explants,disinfection,induction of callus and ad-ventitious buds,proliferation and differentiation,rooting culture were analyzed,and it's seedling transplanting and tissue culturing problems of Iris were systematically summarized.The existing problems in tissue culture of Iris were put forward,and the prospect of its application was prospected,so as to provide ideas and help for tissue cul-ture,establishment of callus system and industrial seedling cultivation of Iris.
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