Identification and Expression Pattern Analysis of 14-3-3 Gene Family in Camellia sinensis
Plant 14-3-3 proteins,also called general regulatory factors(GRFs),are encoded by a large multi-gene family and play a key role in regulating plant growth and development and stress responses.In this study,the tea plant(Camellia sinensis)genome data and successfully identified the tea plant 14-3-3 gene,and bioinformatics analysis was conducted on the protein physicochemical properties,subcellular location,evolutionary relationship,conserved domains and chromosome location of the members of this gene family.The transcriptome data of the 14-3-3 members of tea plant under drought stress and salt stress induced by PEG were analyzed.The results showed that the 14-3-3 gene of tea plant has 23 family members,the relative molecular mass is between 26 460.00~32 852.02,the isoelectric point is in the range of 4.67~5.91,and the subcellular location is shown in the cell mem-brane and cytoplasm;according to system evolution the tree divides the tea plant 14-3-3 gene family divided into ε class and non-ε class;the conserved domain and motif analysis revealed the gene structure of the same subfami-ly,and the conserved domains all belong to the 14-3-3 gene family;gene expression analysis showed that 14-3-3 gene has a higher expression level in flower and fruit tissues;different 14-3-3 genes are differentially expressed under PEG-induced drought stress,tea plant 14-3-3 gene it is widely involved in the growth and development of tea trees and plays an important role in responding to abiotic stress.This study provides a reference for further re-vealing 14-3-3 in the growth and development of tea plant and in response to stress.