首页|茶树14-3-3基因家族的鉴定和表达模式分析

茶树14-3-3基因家族的鉴定和表达模式分析

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植物14-3-3蛋白,也称为通用调节因子(GRFs),由一个大型多基因家族编码,在调节植物生长发育和逆境反应中起着关键作用.本研究通过对茶树基因组数据进行分析,成功的从中鉴定到了茶树14-3-3基因,并对该基因家族成员的蛋白理化性质、亚细胞定位、进化关系、保守结构域、染色体定位等进行生物信息学分析,同时分析了茶树14-3-3成员在PEG诱导的干旱胁迫和盐胁迫处理中的转录组数据.结果表明:茶树14-3-3基因有23个家族成员,相对分子质量在26 460.00~32 852.02之间,等电点在4.67~5.91之间,亚细胞定位显示在细胞膜和细胞质中;根据系统进化树将茶树14-3-3基因家族分为ε类和非ε类;保守结构域和motif分析发现相同亚家族的基因结构,保守结构域都属于14-3-3基因家族;基因表达分析显示,14-3-3基因在花和果实组织中具有较高的表达水平;不同的14-3-3基因在PEG诱导的干旱胁迫下存在差异表达,茶树14-3-3基因广泛参与茶树生长发育和响应非生物胁迫并在其中发挥重要作用,本研究为进一步揭示14-3-3在茶树生长发育以及抗逆响应中提供参考依据.
Identification and Expression Pattern Analysis of 14-3-3 Gene Family in Camellia sinensis
Plant 14-3-3 proteins,also called general regulatory factors(GRFs),are encoded by a large multi-gene family and play a key role in regulating plant growth and development and stress responses.In this study,the tea plant(Camellia sinensis)genome data and successfully identified the tea plant 14-3-3 gene,and bioinformatics analysis was conducted on the protein physicochemical properties,subcellular location,evolutionary relationship,conserved domains and chromosome location of the members of this gene family.The transcriptome data of the 14-3-3 members of tea plant under drought stress and salt stress induced by PEG were analyzed.The results showed that the 14-3-3 gene of tea plant has 23 family members,the relative molecular mass is between 26 460.00~32 852.02,the isoelectric point is in the range of 4.67~5.91,and the subcellular location is shown in the cell mem-brane and cytoplasm;according to system evolution the tree divides the tea plant 14-3-3 gene family divided into ε class and non-ε class;the conserved domain and motif analysis revealed the gene structure of the same subfami-ly,and the conserved domains all belong to the 14-3-3 gene family;gene expression analysis showed that 14-3-3 gene has a higher expression level in flower and fruit tissues;different 14-3-3 genes are differentially expressed under PEG-induced drought stress,tea plant 14-3-3 gene it is widely involved in the growth and development of tea trees and plays an important role in responding to abiotic stress.This study provides a reference for further re-vealing 14-3-3 in the growth and development of tea plant and in response to stress.

Camellia sinensis(L.)14-3-3 gene familyGenome identificationGene expression analysis

安海丽、张宝会、姚新转、吕立堂

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贵州大学生命科学学院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳,550025

贵州大学茶学院,贵阳,550025

茶树 14-3-3基因家族 基因组鉴定 基因表达分析

贵州省茶产业技术创新中心贵州省优秀青年科技人才培养项目贵州省科技计划项目

黔科中引地[2017]4005黔科合平台人才[2019]5651黔科合支撑[2021]一般111

2024

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2024.22(20)