Cloning and Expression Analysis of Small Heat Shock Protein Gene Sms-HSP20 from Eggplant under High Temperature Stress
The ORF(open reading frame)sequence of eggplant SmsHSP20(gene number＞Smechr0302276.1)was searched in Hangzhou-eggplant database,and the specific primers and fluorescence quantitative real-time PCR primers were designed according to this sequence.This gene,encoded 237 amino acids with 714 bp,was cloned from the cDNA of eggplant variety'AC013'leaves,and harbored a HSP20 domain.Bioinformatics analysis of SmsHSP20 revealed that SmsHSP20 protein had high homology with Solanaceae species including potato,pepper,tomato and Chinese wolfberry.Sequence analysis of the SmsHSP20 promoter revealed that it contained two light-responsive cis-elements,multiple plant hormones responsive cis-elements and multiple transcription factor binding sites.The RT-qPCR assay was performed to detect the expression pattern analysis of SmsHSP20 in egg-plant variety'AC013'leaves in the condition of high temperature stress and the result showed that the transcript ex-pression level of SmsHSP20 was sharply up-regulated to peak at 0.5 hours post treatment,indicating that SmsHSP20 may act a positive role in eggplant response to high temperature stress.
EggplantHSP20High temperature stressBioinformatics analysisRT-qPCR
万方数据
维普
