Identification and Bioinformatics Analysis of KNOX1 Gene Family in Brassica
The KNOX gene family is a transcription factor that encodes homeobox proteins and plays an impor-tant regulatory role in plant growth and morphogenesis.The homeobox genes(Class 1 KNOTTED-like homeobox,KNOX1),which one of the classifications,have the function in regulating and maintaining the activity of shoot apical meristem,especially for leaf development and phenotypic formation.In order to explore the role of KNOX 1 in the development of different tissues in Brassica plants,this study used bioinformatics analysis to identify the whole genome KNOX1 family members of some Brassica plants,and analyzed their gene structure,gene expression,conserved domain and phylogenetic relationship.The results showed that a total of 37 KNOX1 genes were obtained in Brassica napus,Brassica rapa,Brassica oleracea,Brassica juncea and Brassica nigra,all of which encoded unstable hydrophilic proteins,and all members contained KNOX1,KNOX2,ELK and Homeobox KN characteristic conserved domains.According to evolutionary analysis and gene structure analysis,it was found that the interspecific homology of Brassica plant KNOX1 proteins was very high,while the KNOX1 site did not fully conform to the triploid evolution theory.Gene expression pattern analysis showed that the expression levels of KNOX1 genes in different Brassica plants were different in roots,stems,leaves,flowers and seeds.This study will provide a theoretical basis for further revealing the specific function of KNOX1 gene in Brassica plants.
万方数据
维普
