Identification and Expression Analysis of Sucrose Synthase(SUS)and Suc-rose Phosphate Synthase(SPS)Genes in Kiwifruit
Sucrose synthase(SUS)and sucrose phosphate synthase(SPS)genes are key enzymes in sucrose metabolism in higher plants.To study the roles of SUS and SPS genes in the sucrose metabolism in kiwifruit(Actinidia chinensis),we used the sequenced genomes'Hongyang'and'White'for genome-wide research,inclu-ding gene identification and genetic structure analysis,phylogenetic relationships detection,conservative domain analysis,chromosome located and genetic evolution pattern analysis.Transcriptome data was used to analyze the expression patterns of SUS and SPS genes.The results showed that there were eight SUS genes(AchSUS1～AchSUS8)and five SPS genes(AchSPS1～AchSPS5)in kiwifruit genome.The SUS family genes were divided into three subfamilies(SUS Ⅰ,SUS Ⅱ and SUS Ⅲ).The SPS family members were divided into four groups(SPSA,SPSB,SPSC and SPSD).Members of the SUS and SPS genes were unevenly located on 11 chromosomes.Seven pairs of paralogous genes underwent fragment duplication and whole genome duplication events,respectively.SUS members in subfamily SUS Ⅲ expanded and were preserved some function,which undergone purified selection during evolution.Expression analysis showed that all SPS gene members and four SUS genes were significantly different expressed in'Hongyang'and'White'.The AchSUS7 gene was only detected expression in'Hongyang'.These genes may play important roles in the sugar accumulation and fruit quality of kiwifruit.
万方数据
维普
