Identification and Expression Analysis of Genes Encoding UGT Involved in the Biosynthesis of Flavonoids in Camellia sinensis
Flavonoids are the most important secondary metabolites in Camellia sinensis,which are responsible for the flavor and healthful functions of tea.Uridine diphosphate glycosyltransferase(UGT)catalyzes the glycosyla-tion of flavonoids,which is a key step in flavonoid synthesis.Therefore,UGT-genes regulate the contents of flavo-noids,and have important research values.In this work,genes encoding UGT-glycosyltransferases in Camellia sinensis were obtained through Iso-seq full-length transcriptome analysis,and UGT genes involved in flavonoid biosynthesis were identified by sequence alignment of UGT genes in Arabidopsis thaliana and the known genes in-volved in synthesis of flavonoids.The expressions of candidated genes and the contents of flavonoids were also de-tected by RT-qPCR and high performance liquid chromatography,then the correlation between gene expressions and the contents of flavonoids were further analyzed.The results revealed that expression of UDP75L121,UDP-75L123,UDP78A15,and UDP94P11 were positively correlated with the contents of catechin gallate(CG)and epi-catechin gallate(ECG);and expression of UDP78A15 was positively correlated with the contents of anthocyanins cyanidin(Cy),pelargonidin(Pel)and malvidin(Ma),while UDP75L123 and UDP78A15 were negatively correlat-ed with the flavanol kaempferol(Kae)and phenolic acid ellagic acid(EA)contents(P＜0.05).The results indicat that these four genes might be involved in the biosynthesis of flavonoids in Camellia sinensis,which provided a ba-sis for further study the biosynthesis of flavonoids in Camellia sinensis.
万方数据
维普
