Flavonoids are the most important secondary metabolites in Camellia sinensis,which are responsible for the flavor and healthful functions of tea.Uridine diphosphate glycosyltransferase(UGT)catalyzes the glycosyla-tion of flavonoids,which is a key step in flavonoid synthesis.Therefore,UGT-genes regulate the contents of flavo-noids,and have important research values.In this work,genes encoding UGT-glycosyltransferases in Camellia sinensis were obtained through Iso-seq full-length transcriptome analysis,and UGT genes involved in flavonoid biosynthesis were identified by sequence alignment of UGT genes in Arabidopsis thaliana and the known genes in-volved in synthesis of flavonoids.The expressions of candidated genes and the contents of flavonoids were also de-tected by RT-qPCR and high performance liquid chromatography,then the correlation between gene expressions and the contents of flavonoids were further analyzed.The results revealed that expression of UDP75L121,UDP-75L123,UDP78A15,and UDP94P11 were positively correlated with the contents of catechin gallate(CG)and epi-catechin gallate(ECG);and expression of UDP78A15 was positively correlated with the contents of anthocyanins cyanidin(Cy),pelargonidin(Pel)and malvidin(Ma),while UDP75L123 and UDP78A15 were negatively correlat-ed with the flavanol kaempferol(Kae)and phenolic acid ellagic acid(EA)contents(P＜0.05).The results indicat that these four genes might be involved in the biosynthesis of flavonoids in Camellia sinensis,which provided a ba-sis for further study the biosynthesis of flavonoids in Camellia sinensis.
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