Optimization of Agrobacterium-mediated Transformation System of Maize Inbred Line B104
To improve the efficiency of maize genetic transformation,here,the young embryos of maize inbred line B104 were used as the receptor,β-glucuronidase gene(GUS)was used as the reporter gene,and the transient expres-sion rate of GUS gene as the index.The transient expression efficiency of GUS gene and its effect of callus induction were observed by treating maize young embryos with different temperatures,different relative centrifuge force(RCF)and different MS salt concentrations in co-culture medium;the callus formation and seedling formation were ob-served in maize young embryos and callus tissue treated with different concentrations of silver nitrate and 6-BA,re-spectively.The results showed that:(1)When the heat shock pretreatment temperature was 43 ℃,the transient expres-sion efficiency of GUS gene could reach the best,and the callus formation rate was 100％;(2)10 000 RCF treatment had effects on GUS transient expression rate and callus induction rate in the scutellum region,which could effectively improve the genetic transformation efficiency of maize young embryos;(3)The formation of callus was not affected by 50％MS salt concentration in co-culture medium,and the transient expression of GUS was better;(4)The hor-mone content of 6-BAP significantly promoted callus formation and seedling regeneration;(5)Silver nitrate sig-nificantly inhibited callus formation in young maize embryos,but significantly promoted seedling formation.This study provided a scientific basis for further optimizing the genetic transformation efficiency of maize inbred lines.
万方数据
维普
