Protein Kinase GmCIPK24 Positively Regulates Salt Tolerance in Soybean
The CBL-CIPK signaling pathway is composed of calcineurin B-like proteins(CBLs)and CBL-inter-acting protein kinases(CIPKs),which play important roles in plant environmental stress responses.However,the biological functions of CBL-CIPK signaling pathway in legume crops salt stress response remain unclear.In this study,a CIPK protein encoding gene,GmCIPK24(Glyma.17G113700),was cloned from soybean cultivar'Willia-ms 82'.Sequence analysis showed that the open reading frame of GmCIPK24 was 1 341 bp,encoding 427 amino acids.Phylogenetic tree assay demonstrated that GmCIPK24 belonged to the intron-rich CIPK protein kinase group.Protein structure analysis illustrated that GmCIPK24 had a Ser/Thr kinase domain and a conserved NAF/FISL mo-tif.The GmCIPK24 promoter sequence had cis-acting elements related to salt stress,ABA,and drought stress.Fur-thermore,RT-qPCR assays illustrated that the expression of GmCIPK24 was induced by salt stress,abscisic acid(ABA),alkaline stress,and osmotic stress.Overexpression of GmCIPK24 significantly improved the salt tolerance of transgenic soybean plants.These results suggest that GmCIPK24 plays an important role in improving soybean salt tolerance.Furthermore,this study provides basic information in elucidating the role of CBL-CIPK signaling pathway in soybean salt stress response and soybean salt tolerance breeding.
万方数据
维普
